Coupling in vitro food digestion with in vitro epithelial absorption; recommendations for biocompatibility

Kondrashina, Alina; Arranz Gutiérrez, Elena María; Cilla Tatay, Antonio; Faria, Miguel A.; Santos-Hernández, Marta; Miralles Buraglia, Beatriz; Hashemi, Negin; Rasmussen, Martin Krøyer; Young, Jette F.; Barberá Sáez, Reyes; Mamone, Gianfranco; Tomás-Cobos, Lidia; Bastiaan-Net, Shanna; Corredig, Milena; Giblin, Linda

doi:10.1080/10408398.2023.2214628

Coupling in vitro food digestion with in vitro epithelial absorption; recommendations for biocompatibility

Kondrashina, Alina ²
Arranz Gutiérrez, Elena María ³
Cilla Tatay, Antonio ⁵
Faria, Miguel A. ⁹
Santos-Hernández, Marta ⁴
Miralles Buraglia, Beatriz ¹⁰
Hashemi, Negin ¹
Rasmussen, Martin Krøyer ¹
Young, Jette F. ¹
Barberá Sáez, Reyes ⁵
Mamone, Gianfranco ⁸
Tomás-Cobos, Lidia ¹¹
Bastiaan-Net, Shanna ⁷
Corredig, Milena ¹
Giblin, Linda ⁶

1 Department of Food Science, Aarhus University, Agro Food Park 48, Aarhus, Denmark
2 Global Research and Technology Centre, H&H Group, H&H Research, Fermoy, Ireland
3 Department of Nutrition and Food Science, Faculty of Pharmacy, Complutense University of Madrid (UCM), Madrid, Spain
4 Wellcome Trust - MRC Institute of Metabolic Science, Metabolic Research Laboratories, Addenbrooke’s Hospital, Cambridge, UK
5 Nutrition and Food Science Area, Faculty of Pharmacy, University of Valencia, Valencia, Spain
6 Teagasc Food Research Centre, Moorepark, Fermoy, Co Cork, Ireland
7 Wageningen Food & Biobased Research, Wageningen University & Research, WG Wageningen, The Netherlands
8 Institute of Food Sciences - National Research Council, Avellino, Italy
9 LAQV/REQUIMTE, Laboratório de Bromatologia e Hidrologia, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade do Porto, Rua de Jorge Viterbo Ferreira, 228, Porto, Portugal
10 Institute of Food Science Research CIAL (CSIC-UAM), Madrid, Spain
11 In vitro preclinical studies department, AINIA, Avenida Benjamín Franklin 5-11, Parque Tecnológico de Valencia, Paterna, Spain

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Revista:

Critical Reviews in Food Science and Nutrition

ISSN: 1040-8398, 1549-7852

Año de publicación: 2023

Páginas: 1-19

Tipo: Artículo

DOI: 10.1080/10408398.2023.2214628 GOOGLE SCHOLAR Acceso abierto editor

Otras publicaciones en: Critical Reviews in Food Science and Nutrition

Objetivos de desarrollo sostenible

Resumen

As food transits the gastrointestinal tract, food structures are disrupted and nutrients are absorbed across the gut barrier. In the past decade, great efforts have focused on the creation of a consensus gastrointestinal digestion protocol (i.e., INFOGEST method) to mimic digestion in the upper gut. However, to better determine the fate of food components, it is also critical to mimic food absorption in vitro. This is usually performed by treating polarized epithelial cells (i.e., differentiated Caco-2 monolayers) with food digesta. This food digesta contains digestive enzymes and bile salts, and if following the INFOGEST protocol, at concentrations that although physiologically relevant are harmful to cells. The lack of a harmonized protocol on how to prepare the food digesta samples for downstream Caco-2 studies creates challenges in comparing inter laboratory results. This article aims to critically review the current detoxification practices, highlight potential routes and their limitations, and recommend common approaches to ensure food digesta is biocompatible with Caco-2 monolayers. Our ultimate aim is to agree a harmonized consensus protocol or framework for in vitro studies focused on the absorption of food components across the intestinal barrier.

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Financiadores

Science Foundation Ireland

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