Commensal Staphylococcus isolates from the nasal cavity of community older adults in Valencia (Spain) and their resistance to methicillin and other antibiotics

  1. Gozalbo Falomir, Daniel 1
  2. Rico Vidal, Hortensia 1
  3. Falomir Llorens, María Pilar 1
  1. 1 Universitat de València
    info

    Universitat de València

    Valencia, España

    ROR https://ror.org/043nxc105

Revue:
European Journal of Health Research: (EJHR)

ISSN: 2444-9067,  2445-0308

Année de publication: 2019

Titre de la publication: (Diciembre, 2019)

Volumen: 5

Número: 2

Pages: 145-158

Type: Article

DOI: 10.30552/EJHR.V5I2.185 DIALNET GOOGLE SCHOLAR lock_openDialnet editor

D'autres publications dans: European Journal of Health Research: (EJHR)

Objectifs de Développement Durable

Résumé

Las cepas de Staphylococcus aureus resistentes a meticilina (SARM) están ampliamente diseminadas, causando infecciones hospitalarias y comunitarias, y los portadores sanos de SARM constituyen un reservorio del patógeno. Otras especies de estafilococos coagulasa negativos (CoNS) colonizan animales y humanos, e incluyen cepas resistentes a meticilina (CoNSRM). En este trabajo hemos determinado la prevalencia de S. aureus y CoNS en la cavidad nasal de adultos mayores sanos (n= 27, edad media: 63.7 años) y su resistencia a meticilina y otros antibióticos. Se obtuvieron 35 aislados de Staphylococcus. Todos los individuos (100%) portaban al menos una cepa de Staphylococcus; el 15% eran portadores de S. aureus, y en ocho sujetos (30%) se aislaron dos cepas. La resistencia a meticilina fue del 25% y del 35% para los aislados de S. aureus y CoNS, respectivamente. La mayoría de aislados fueron resistentes a penicilina G (90%) y claritromicina (45%). Otras resistencias fueron menos frecuentes (rifampicina, tetraciclina, fosfomicina, ciprofloxacino) y no se encontraron resistencias a cloranfenicol ni vancomicina. Se detectaron aislados multirresistentes a tres o cuatro quimioterápicos (20% de aislados). Estos resultados sugieren que la cavidad nasal puede constituir un nicho para la transferencia de resistencias entre estafilococos, y que la vigilancia epidemiológica debe incluir tanto a los portadores de SARM como de CoNSMR.

Références bibliographiques

  • Argudín, M.A., Mendoza, M.C., and Rodicio, M.R. (2010). Food poisoning and Staphylococcus aureus enterotoxins. Toxins, 2, 1751-1773. doi:10.3390/toxins2071751
  • Baquero, F. (2012). Metagenomic epidemiology: a public health need for the control of antimicrobial resistance. Clinical Microbiology and Infection, 18(Suppl. 4), 67-73. doi:10.1111/j.1469-0691.2012.03860.x
  • Barbier, F., Ruppé, E., Hernandez, D., Lebaux, D., Francois, P., Felix, B,… Ruimy, R. (2010). Methicillin-resistent coagulase-negative staphylococci in the community: high homology of SSCmec IVa between Staphylococcus epidermidis and major clones of methicillinresistant Staphylococcus aureus. The Journal of Infectious Diseases, 202(2), 270-281. doi:10.1086/653483
  • Becker, A., Heilmann, C., and Peters, G. (2014). Coagulase-negative staphylococci. Clinical Microbiology Reviews, 27(4), 870-926. doi:10.1128/CMR.00109-13
  • Bond, R., and Loeffler, A. (2012). What’s happened to Staphylococcus intermedius? Taxonomic emergence of multi-drug resistance. Journal of Small Animals Practice, 53(3), 147-154. doi:10.1111/j.1748-5827.2011.01165.x
  • Cercenado, E. (2016). Epidemiology of the infection by resistant Gram-positive microorganisms. Revista Española de Quimioterapia, 26(Suppl. 1), 6-9.
  • Cuevas, O., Cercenado, E., Vindel, A., Guinea, J., Sánchez-Conde, M., Sánchez-Somolinos, M., and Bouza, E. (2004). Evolution of the antimicrobial resistance of Staphylococcus spp. in Spain: five nationwide prevalence studies, 1986 to 2002. Antimicrobial Agents and
  • Chemotherapy, 48(11), 4240-4245. doi:10.1128/AAC.48.11.4240-4245.2004
  • Couto, N., Belas, A., Kadlec, K., Schwarz, S., and Pomba, C. (2015). Clonal diversity, virulence patterns and antimicrobial and biocide susceptibility among human, animal and environmental MRSA in Portugal. Journal of Antimicrobial Chemotherapy, 70, 2483- 2487. doi:10.1093/jac/dkv141
  • Doulgeraki, A.I., Di Ciccio, P., Ianeri, A., and Nychas, G.J.E. (2017). Methicillin-resistant foodrelated Staphylococcus aureus: a review of current knowledge and biofilm formation for future studies and applications. Research in Microbiology, 168, 1-15. doi:10.1016/j.resmic.2016.08.001
  • Dulon, M., Haamann, F., Peters, C., Schablon, A., and Nienhaus, A (2011). MRSA prevalence in European healthcare settings: a review. BMC Infectious Diseases, 11, 138-151. doi:10.1186/1471-2334-11-138
  • EPINE-EPPS (2017). Estudio de prevalencia de las infecciones nosocomiales en España. Madrid: Sociedad Española de Medicina Preventiva, Salud Pública e Higiene.
  • Falomir, M.P., Gozalbo, D., and Rico, H. (2014). Occurrence of methicillin-resistant Staphylococcus aureus in the nasal cavity of healthy volunteer students of the University of Valencia (Spain). Journal of Microbiology, Immunology and Infection, 47, 162-163. doi:10.1016/j.jmii.2013.05.009
  • Falomir, M.P., Jávega, A., Rico, H., and Gozalbo, D. (2018). Nasal isolates of comensal Staphylococcus aureus and non-aureus species from healthy young adults in Valencia (Spain) and their resistance to chemotherapeutic agents. Annals of Epidemiology and Public Health, 1, 1004. doi:10.33582/2639-4391/1004
  • Fisher, E.L., Otto, M., and Cheung, G.Y.C. (2018). Basis of virulence in enterotoxin-mediated staphylococcal food poisoning. Frontiers in Microbiology, 9, 436. doi:10.3389/fmicb.2018.00436
  • Galán-Sánchez, F., Pérez-Eslava, M., Machuca, J., Trujillo-Soto, T., Arca.Suarez, J., and Rodríguez-Iglesias, M. (2019). Staphylococcus aureus carriage in older populations in community residential care homes: prevalence and molecular characterization of MRSA isolates. Enfermedades Infecciosas y Microbiología Clínica, 37(3), 172-175. doi:10.1016/j.eimc.2018.05.011
  • Gardella, N., Murzicato, S., Di Gregorio, S., Cuirolo, A., Desse, J., Crudo, F,… Mollerach, M. (2011). Prevalence and characterization of methicillin-resistant Staphylococcus aureus among healthy children in a city of Argentina. Infection, Genetics and Evolution, 11(5), 1066-1071. doi:10.1016/j.meegid.2011.03.019
  • Gorwitz, R.J., Kruszon-Moran, D., McAllister, S.K., McQuillan, G., McDougal, L.K., Fosheim, G.E.,… Kuehnert, M.J. (2008). Changes in the prevalence of nasal colonization with Staphylococcus aureus in the United States, 2001-2004. Journal of Infectious Diseases, 197(9), 1226-1234. doi:10.1086/533494
  • Hanssen, A.M., Kjeldsen, G., and Sollid, J.U. (2004). Local variants of staphylococcal cassette chromosome mec in sporadic methicillin-resistant Staphylococcus aureus and methicillin-resistant coagulase-negative staphylococci: evidence of horizontal gene transfer? Antimicrobial Agents and Chemotherapy, 48, 285-296. doi:10.1128/AAC.48.1.285-296.2004
  • Hiramatsu, K., Katayama, Y., Matsuo, M., Sasaki, T., Morimoto, Y, Sekiguchi, A., and Baba, T. (2014). Multi-drug-resistant Staphylococcus aureus and future chemotehrapy. Journal of Infection and Chemotherapy, 20, 593-601. doi:10.1007/s00467-014-2955-8
  • Kadlec, K., and Schwarz, S. (2012). Antimicrobial resistance of Staphylococcus pseudointermedius. Veterinary Dermatology, 23(4), 276-282. doi:10.1111/j.1365- 3164.2012.01056.x
  • Krismer, B., Weidenmaier, C., Zipperer, A., and Peschel, A. (2017). The commensal lifestyle of Staphylococcus aureus and its interactions with the nasal microbiota. Nature Reviews Microbiology, 15(11), 675-687. doi:10.1038/nrmicro.2017.104
  • Lakhundi, S., and Zhang, K. (2018). Methicillin-resistant Staphylococcus aureus: molecular characterization, evolution, and epidemiology. Clinical Microbiology Reviews, 31(4), pii: e00020-18. doi:10.1128/CMR.00020-18
  • Miller, J.G., and Kaplan, S.L. (2009). Staphylococcus aureus: a community pathogen. Infectious Disease Clinics of North America, 23, 35-52. doi:10.1016/j.idc.2008.10.002
  • Otto, M. (2012). MRSA virulence and spread. Cellular Microbiology, 14, 1513-1521. doi:10.1111/j.1462-5822.2012.01832.x
  • Pomba, C., Rantala, M., Greko, C., Baptiste, K.E., Catry, B., van Duijkeren, E.,… Törneke, K. (2017). Public health risk of antimicrobial resistance transfer from companion animals. Journal of Antimicrobial Chemotherapy, 72(4), 957-968. doi:10.1093/jac/dkw481
  • Pujol, M., and Limón, E. (2013). General epidemiology of nosocomial infections. Surveillance systems and programs. Enfermedades Infecciosas y Microbiología Clínica, 31(2), 108- 113. doi:10.1016/j.eimc.2013.01.001
  • Ruscher, C., Lubke-Beckeer. A., Wlekinsli, C.G., Soba, A., Wieler, L.H., and Walther, B. (2009). Prevalence of methicillin-resistant Staphylococcus pseudointermedius isolated from clinical samples of companion animals and equidaes. Veterinary Microbiology, 136, 197-201. doi:10.1016/j.vetmic.2008.10.023
  • Show, A.C., Joshi, S., Greenwood, H., Panda, A., and Lord, J.M. (2012). Aging of the innate immune system. Current Opinion in Immunology, 22, 507-513. doi:10.1016/j.coi.2010.05.003
  • Sievert, D.M., Ricks, P., Edwards, J.R., Schneider, A., Patel, J., Srinivasan, A., … Fridkin, S. (2013). Antimicrobial-resistant pathogens associated with healthcare-associated infections: summary of data reported to the National Healthcare Safety Network at the centers for disease control and prevention, 2009-2010. Infection Control and Hospital Epidemiology, 34, 1-14. doi:10.1086/668770
  • Teira, R., Teira, A., Campo, A.B., and de Benito, I. (2013). Prevalence of nasopharyngeal colonization by methicillin-resistent Staphylococcus aureus in a population of high school students in Torrelavega (Spain). Enfermedades Infecciosas y Microbiología Clínica, 31(5), 349. doi:10.1016/j.eimc.2012.10.006
  • Tong, S.Y.C., Davis, J.S., Eichemberger, E., Holland, T.L., and Fowler, V.C. Jr. (2015). Staphylococcus aureus infections: epidemiology, pathophysiology, clinical manifestations, and management. Clinical Microbiology Reviews, 28, 603-661. doi:10.1128/CMR.00134-14
  • Van Bijnen, E.M.E., Paget, J., de Lange-de Klerk, E.S.M., den Heijer, C.D.J., Versporten, A., Stobberingh, E.E.,… Schellevis, F.G. (2015). Antibiotic exposure and other risk factors for antimicrobial resistance in nasal commensal Staphylococcus aureus: an ecological study in 8 european countries. PLoS ONE, 10(8), e0135093. doi:10.1371/journal.pone.0135094
  • Vindel, A., Trincado, P., Cuevas, O., Ballesteros, C, Bouza, E., and Cercenado, E. (2014). Molecular epidemiology of community-associated methicillin resistant Staphylococcus aureus in Spain: 2004-2012. Journal of Antimicrobial Chemotherapy, 69, 2913-2919. doi:10.1093/jac/dku232
  • Wang, H.K., Huang, C.Y., Chen, C.J., and Huang, Y.C. (2017). Nasal Staphylococcus aureus carriage among college student athletes in northern Taiwan. Journal of Microbiology, Immunology and Infection, 50, 537-540. doi:10.1016/j.jmii.2016.11.005
  • Weidenmaier, C., Goerke, C., and Wolz, C. (2012). Staphylococcus aureus determinants for nasal colonization. Trends in Microbiology, 20, 243-250. doi:10.1016/j.tim.2012.03.004
  • Wertheim, H.F., Vos, M.C., Ott, A., van Belkum, A., Voss, A., Kluytmans, J.A.,… Verbrugh, H.A. (2004). Risk and outcome of nosocomial Staphylococcus aureus bacteraemia in nasal carriers versus non-carriers. Lancet, 364, 703.705. doi:10.1016/S0140- 6736(04)16897-9
La source de données: Dialnet