Aproximaciones inmunoanalíticas para el control de xenobióticos y biotoxinas en alimentos

  1. Mercader, Josep V. 1
  2. Abad-Somovilla, Antonio 2
  3. Agulló, Consuelo 2
  4. Abad-Fuentes, Antonio 1
  1. 1 Instituto de Agroquímica y Tecnología de Alimentos (Valencia). Consejo Superior de Investigaciones Científicas
  2. 2 Departamento de Química Orgánica. Universitat de València
Journal:
Arbor: Ciencia, pensamiento y cultura

ISSN: 0210-1963

Year of publication: 2020

Volume: 196

Issue: 795

Type: Article

DOI: 10.3989/ARBOR.2020.795N1006 DIALNET GOOGLE SCHOLAR lock_openOpen access editor

More publications in: Arbor: Ciencia, pensamiento y cultura

Abstract

Analytical methods are required to effectively ensure food quality and safety. A reliable determination of any harmful substance potentially present in a food product is mandatory before it reaches the consumer. One of the analytical approaches that nowadays contributes to attaining this goal includes a series of techniques that use antibodies as essential elements for the detection of the target analyte, which collectively are known as immunochemical methods. This article aims to provide a basic overview of the biochemical principles underlying these technologies and their benefits and limitations for the determination of chemical contaminants, residues and additives in food matrices. In the last section, some of our initiatives in this field that resulted in commercially available rapid kits following technology transfer to the industrial sector are discussed.

€ View funding

Funding information

Estos estudios fueron financiados con proyectos de diferentes convocatorias p?blicas (GVPRE/2008/236, AICO/2018/111, AGL2006-12750-C02-01/ALI, AGL200912940-C02-01-02/ALI, AGL2012-39965-C02, AGL201564488-C2, PET2006-0423-00, IDI-20140850, RTC-20153348-2) y cofinanciados con fondos FEDER. Nos gustar?a expresar nuestro reconocimiento a todos los estudiantes y t?cnicos que han formado parte de nuestro grupo por su decisiva contribuci?n en la generaci?n de la colecci?n de inmunorreactivos que se describe en este art?culo.

Funders

Bibliographic References

  • Abad-Fuentes, A., Esteve-Turrillas, F. A., Agulló, C., Abad-Somovilla, A. y Mercader, J. V. (2012). Development of competitive enzyme-linked immunosorbent assays for boscalid determination in fruit juices. Food Chemistry, 135 (1), pp. 276‒284.
  • Esteve-Turrillas, F. A., Abad-Somovilla, A., Quiñones-Reyes, G., Agulló, C., Mercader, J. V. y Abad-Fuentes, A. (2015). Monoclonal antibody-based immunoassays for cyprodinil residue analysis in QuEChERS-based fruit extracts. Food Chemistry, 187, pp. 530-536.
  • Esteve-Turrillas, F. A., Mercader, J. V., Agulló, C., Abad-Somovilla, A. y Abad-Fuentes, A. (2015). Moiety and linker site heterologies for highly sensitive immunoanalysis of cyprodinil in fermented alcoholic drinks. Food Control, 50, pp. 393-400.
  • Esteve-Turrillas, F. A., Parra, J., Abad-Fuentes, A., Agulló, C., Abad-Somovilla, A. y Mercader, J. V. (2010). Hapten synthesis, monoclonal antibody generation, and development of competitive immunoassays for the analysis of picoxystrobin in beer. Analytica Chimica Acta, 682 (1-2), pp. 93-103.
  • González-Techera, A., Varell, L., Last, J. A., Hammock, B. D. y González-Sapienza, G. (2007). Phage anti-immune complex assay: General strategy for non-competitive immunodetection of small molecules. Analytical Chemistry, 79 (20), pp. 7799-7806.
  • Landsteiner, K. y Simms, S. (1923). Production of heterogenetic antibodies with mixtures of the binding part of the antigen and protein. Journal of Experimental Medicine, 38 (2), pp. 127-138.
  • López-Moreno, R., Mercader, J. V., Agulló, C., Abad-Somovilla, A. y Abad-Fuentes, A. (2014). Immunoassays for trifloxystrobin analysis. Part I. Rational design of regioisomeric haptens and production of monoclonal antibodies. Food Chemistry, 152, pp. 230‒236.
  • López-Puertollano, D., Mercader, J. V., Agulló, C., Abad-Somovilla, A. y Abad-Fuentes, A. (2018). Novel hapten and monoclonal antibodies with subnanomolar affinity for a classical analytical target, ochratoxin A. Scientific Reports, 8 (1), 9761.
  • Mercader, J. V., Agulló, C., Abad-Somovilla, A. y Abad-Fuentes, A. (2011). Synthesis of site-heterologous haptens for high-affinity anti-pyraclostrobin antibody generation. Organic & Biomolecular Chemistry, 9 (5), pp. 1443-1453.
  • Mercader, J. V., López-Moreno, R., Esteve- Turrillas, F. A., Abad-Somovilla, A. y Abad-Fuentes, A. (2014). Immunoassays for trifloxystrobin analysis. Part II. Assay development and application to residue determination in food. Food Chemistry, 162, pp. 41-46.
  • Montalbetti, C. A. G. N. y Falque, V. (2005). Amide bond formation and peptide coupling. Tetrahedron, 61 (46), pp. 10827- 10852.
  • Parra, J., Mercader, J. V., Agulló, C., Abad- Somovilla, A. y Abad-Fuentes, A. (2012). Generation of anti-azoxystrobin monoclonal antibodies from regioisomeric haptens functionalized at selected sites and development of indirect competitive immunoassays. Analytica Chimica Acta, 715, pp. 105-112.
  • Ramón-Azcón, J., Sánchez-Baeza, F., Sanvicens, N. y Marco, M. P. (2009). Development of an enzyme-linked immunosorbent assay for determination of the miticide bromopropylate. Journal of Agricultural and Food Chemistry, 57 (2), pp. 375-384.
  • Sanvicens, N., Pichon, V., Hennion, M. C. y Marco, M. P. (2003). Preparation of antibodies and development of an enzyme-linked immunosorbent assay for determination of dealkylated hydroxytriazines. Journal of Agricultural and Food Chemistry, 51 (1), pp. 156-164..
  • Suárez-Pantaleón, C., Mercader, J. V., Agulló, C., Abad-Somovilla, A. y Abad-Fuentes, A. (2008). Production and characterization of monoclonal and polyclonal antibodies to forchlorfenuron. Journal of Agricultural and Food Chemistry, 56 (23), pp. 11122‒11131.
  • Suárez-Pantaleón, C., Mercader, J. V., Agulló, C., Abad-Somovilla, A. y Abad- Fuentes, A. (2011). Forchlorfenuron-mimicking haptens: from immunogen design to characterization by hierarchical clustering analysis. Organic & Biomolecular Chemistry, 9 (13), pp. 4863-4872.
  • Vallejo, R. P., Bogus, E. R. y Mumma, R. O. (1982). Effects of hapten structure and bridging groups on antisera specificity in parathion immunoassay development. Journal of Agricultural and Food Chemistry, 30 (3), pp. 572-580.
Data source: Dialnet