Estudio comparativo de la diversidad genética de Mycobacterium tuberculosis complex mediante análisis de polimorfismo de longitud de fragmentos amplificados y número variable de repeticiones en tándem de unidades repetitivas interespaciadas de micobact

  1. Jiménez Arias, Ana Patricia
  2. Lahiguera, María José
  3. Borrás, Rafael
  4. Cardona, Concepción Gimeno
  5. Grijalva Silva, Marcelo
  6. Vallejo López, María José
  7. Guna Serrano, María del Remedio
Revista:
Revista Ecuatoriana de Medicina y Ciencias Biológicas: REMCB

ISSN: 2477-9148,  2477-9113

Año de publicación: 2018

Volumen: 39

Número: 1

Páginas: 63-71

Tipo: Artículo

DOI: 10.26807/REMCB.V39I1.568 DIALNET GOOGLE SCHOLAR lock_openDialnet editor

Otras publicaciones en: Revista Ecuatoriana de Medicina y Ciencias Biológicas: REMCB

Resumen

Las especies dentro del complejo Mycobacterium tuberculosis (MTB) son genéticamente monomórficas, por lo tanto existe una gran  necesidad de métodos confiables de genotipificación para la comprensión de la epidemiologia de esta enfermedad. Esta investigación evalúa la diversidad genética de una colección española de ochenta y tres aislados mediante el uso de polimorfismo de  longitud de  fragmentos amplificados (AFLP) y número variable de repeticiones en tándem de unidades repetitivas interespaciadas (15-loci MIRU-VNTR). Los resultados obtenidos mostraron 7 patrones de AFLP (P1-P7) cuyos coeficientes de Dice fueron: 71% para P1 vs P7 y 96% para P1 vs P2 y P2 vs P4. MIRU-VNTR demostró 25 patrones únicos y 14 clusters. Los linajes encontrados fueron: Haarlem (23, 36.51%), Cammeroon (2, 3.17%), LAM (12, 19.05%), West African (6, 9.52%) y EAI (1, 1.59%). Los índices de discriminación para AFLP fueron de 0.61 y  0.92 para MIRU-VNTR. En conclusión, este estudio demostró que MIRU-VNTRes robusto y reproducible para genotificar MTB. Adicionalmente, AFLP simplificado es relativamente sencillo de realizar y puede ser útil en el análisis de aislados con recursos limitados

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Fuente de los datos: Dialnet