Estudio comparativo de la diversidad genética de Mycobacterium tuberculosis complex mediante análisis de polimorfismo de longitud de fragmentos amplificados y número variable de repeticiones en tándem de unidades repetitivas interespaciadas de micobact

  1. Jiménez Arias, Ana Patricia
  2. Lahiguera, María José
  3. Borrás, Rafael
  4. Cardona, Concepción Gimeno
  5. Grijalva Silva, Marcelo
  6. Vallejo López, María José
  7. Guna Serrano, María del Remedio
Journal:
Revista Ecuatoriana de Medicina y Ciencias Biológicas: REMCB

ISSN: 2477-9148,  2477-9113

Year of publication: 2018

Volume: 39

Issue: 1

Pages: 63-71

Type: Article

DOI: 10.26807/REMCB.V39I1.568 DIALNET GOOGLE SCHOLAR lock_openDialnet editor

More publications in: Revista Ecuatoriana de Medicina y Ciencias Biológicas: REMCB

Abstract

Species within the Mycobacterium tuberculosis (MTB) Complex are genetically monomorphic, hence the need of genotyping methods for a comprehensive understanding of the epidemiology of the disease. The genetic diversity of a Spain collection of eighty-three, GenoType MTBC® -confirmed Mycobacterium tuberculosis clinical isolates was assessed by simplified AFLP and 15-loci MIRU-VNTR. AFLP results showed 7 patterns (P1-P7), Dice´s coefficient was 71% for P1 vs P7 and 96% for P1 vs P2 and P2 vs P4. MIRU-VNTR showed 25 unique patterns and 14 clusters. Lineages found were as follows: Haarlem (23, 36.51%), Cammeroon (2, 3.17%), LAM (12, 19.05%), West African (6, 9.52%) and EAI (1, 1.59%). Discrimination indexes were 0.61 for AFLP and 0.92 for MIRU-VNTR. In conclusion,  MIRU-VNTR is robust and reproducible for MTB genotyping. Simplified AFLP is a relatively easy-to-perform approach that might be useful for screening of isolates or in low resource settings.

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