COP1 destabilizes DELLA proteins in <i>Arabidopsis</i>

  1. Blanco-Touriñán, Noel 3
  2. Legris, Martina 6
  3. Minguet, Eugenio G. 38
  4. Costigliolo-Rojas, Cecilia 6
  5. Nohales, María A. 9
  6. Iniesto, Elisa 1
  7. García-León, Marta 1
  8. Pacín, Manuel 7
  9. Heucken, Nicole 2
  10. Blomeier, Tim 2
  11. Locascio, Antonella 3
  12. Černý, Martin 5
  13. Esteve-Bruna, David 3
  14. Díez-Díaz, Mónica 4
  15. Brzobohatý, Břetislav 5
  16. Frerigmann, Henning 10
  17. Zurbriggen, Matías D. 2
  18. Kay, Steve A. 9
  19. Rubio, Vicente 1
  20. Blázquez, Miguel A. 3
  21. Casal, Jorge J. 67
  22. Alabadí, David 3
  1. 1 Departamento de Genética Molecular de Plantas, Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas, 28049 Madrid, Spain;
  2. 2 Institute of Synthetic Biology and Cluster of Excellence in Plant Sciences, University of Düsseldorf, 40225 Düsseldorf, Germany;
  3. 3 Instituto de Biología Molecular y Celular de Plantas, Consejo Superior de Investigaciones Científicas, Universidad Politécnica de Valencia, 46022 Valencia, Spain;
  4. 4 Facultad de Veterinaria y Ciencias Experimentales, Universidad Católica de Valencia, 46001 Valencia, Spain;
  5. 5 Department of Molecular Biology and Radiobiology, Central European Institute of Technology, Faculty of AgriSciences, Mendel University in Brno, CZ-61300 Brno, Czech Republic;
  6. 6 Fundación Instituto Leloir, Instituto de Investigaciones Bioquímicas de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas, 1405 Buenos Aires, Argentina;
  7. 7 Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura, Facultad de Agronomía, Universidad de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas, 1417 Buenos Aires, Argentina;
  8. 8 Departament de Biologia Vegetal, Facultat de Ciències Biològiques, Universitat de València
  9. 9 Department of Neurology, Keck School of Medicine, University of Southern California, Los Angeles, CA 90098;
  10. 10 Department of Plant Microbe Interactions and Cluster of Excellence in Plant Sciences, Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany
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Zeitschrift:
Proceedings of the National Academy of Sciences

ISSN: 0027-8424,  1091-6490

Datum der Publikation: 2020

Ausgabe: 117

Nummer: 24

Seiten: 13792-13799

Art: Artikel

DOI: 10.1073/PNAS.1907969117 GOOGLE SCHOLAR lock_openOpen Access editor

Andere Publikationen in: Proceedings of the National Academy of Sciences

Zusammenfassung

DELLA transcriptional regulators are central components in the control of plant growth responses to the environment. This control is considered to be mediated by changes in the metabolism of the hormones gibberellins (GAs), which promote the degradation of DELLAs. However, here we show that warm temperature or shade reduced the stability of a GA-insensitive DELLA allele in Arabidopsis thaliana. Furthermore, the degradation of DELLA induced by the warmth preceded changes in GA levels and depended on the E3 ubiquitin ligase CONSTITUTIVELY PHOTOMORPHOGENIC1 (COP1). COP1 enhanced the degradation of normal and GA-insensitive DELLA alleles when coexpressed in Nicotiana benthamiana. DELLA proteins physically interacted with COP1 in yeast, mammalian, and plant cells. This interaction was enhanced by the COP1 complex partner SUPRESSOR OF phyA-105 1 (SPA1). The level of ubiquitination of DELLA was enhanced by COP1 and COP1 ubiquitinated DELLA proteins in vitro. We propose that DELLAs are destabilized not only by the canonical GA-dependent pathway but also by COP1 and that this control is relevant for growth responses to shade and warm temperature.

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Informationen zur Finanzierung

Geldgeber

  • Spanish Ministry of Economy, Industry and Competitiveness and AEI/FEDER/EU
    • BIO2016-79133-P
  • Spanish Ministry of Economy, Industry and Competitiveness and AEI/FEDER/EU
    • BIO2013-46539-R
  • Spanish Ministry of Economy, Industry and Competitiveness and AEI/FEDER/EU
    • BIO2016-80551-R
  • European Union
    • H2020-MSCA-RISE-2014-644435
  • Argentinian Agencia Nacional de Promoción Científica y Tecnológica
    • PICT-2016-1459
  • Universidad de Buenos Aires
    • 20020170100505BA
  • National Institute of General Medical Sciences of the National Institutes of Health
    • RO1GM067837
  • National Institute of General Medical Sciences of the National Institutes of Health
    • RO1GM056006
  • German Research Foundation
    • 390686111
  • MEYS of the Czech Republic
    • LQ1601

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