Comparative genomics and evolutionary analysis of Lactococcus garvieae isolated from human endocarditis

  1. Francés Cuesta, Carlos 345
  2. Ansari, Iván 345
  3. Fernández-Garayzábal Fernández, José Francisco 12
  4. Gibello Prieto, Alicia 2
  5. González Candelas, Fernando 345
  1. 1 VISAVET Animal Health Surveillance Center, Complutense University, Madrid, Spain
  2. 2 Department of Animal Health, Faculty of Veterinary Sciences, Complutense University
  3. 3 Joint Research Unit Infection and Public Health FISABIO-University of Valencia
  4. 4 Institute for Integrative Systems Biology (I2SysBio, UV-CSIC)
  5. 5 CIBER in Epidemiology and Public Health, Valencia, Spain
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Revista:
Microbial Genomics

ISSN: 2057-5858

Año de publicación: 2022

Volumen: 8

Número: 2

Tipo: Artículo

DOI: 10.1099/MGEN.0.000771 GOOGLE SCHOLAR lock_openAcceso abierto editor

Otras publicaciones en: Microbial Genomics

Resumen

Lactococcus garvieae is a well-known pathogen of fish, but is rarely involved in infections in humans and other mammals. In humans, the main clinical manifestation of L. garvieae infections is endocarditis usually related to the ingestion of contaminated food, such as undercooked fish and shellfish. This study presents the first complete genomic sequence of a clinical L. garvieae strain isolated from a patient with endocarditis and its comparative analysis with other genomes. This human isolate contains a circular chromosome of 2 099 060 bp and one plasmid of 50 557 bp. In comparison with other fully sequenced L. garvieae strains, the chromosomal DNA of L. garvieae Lg-Granada carries a low proportion of insertion sequence elements and a higher number of putative prophages. Our results show that, in general, L. garvieae is a highly recombinogenic species with an open pangenome in which almost 30 % of its genome has undergone horizontal transfers. Within the genus Lactococcus, L. lactis is the main donor of genetic components to L. garvieae but, taking Lg-Granada as a representative, this bacterium tends to import more genes from Bacilli taxa than from other Lactococcus species.

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Referencias bibliográficas

  • 10.2903/j.efsa.2020.5965
  • 10.1016/j.vetmic.2013.09.011
  • 10.3168/jds.2015-9579
  • 10.1097/MD.0000000000013658
  • 10.1016/j.jiac.2018.07.010
  • 10.3354/dao03083
  • 10.3947/ic.2019.0015
  • 10.1111/j.1742-1241.2006.00855.x
  • 10.1099/00207713-46-3-664
  • 10.1111/j.1472-765X.2011.03153.x
  • 10.1128/JCM.02369-12
  • 10.1016/j.rvsc.2016.09.010
  • 10.1177/2324709619832052
  • 10.1186/s12879-019-3912-8
  • 10.1111/j.1365-2672.2006.03265.x
  • 10.1016/j.ijfoodmicro.2007.11.014
  • 10.1016/j.ijfoodmicro.2009.09.023
  • 10.1371/journal.pone.0126101
  • 10.1016/j.nmni.2015.02.001
  • 10.1038/nmeth.2474
  • 10.1126/science.287.5461.2196
  • 10.1186/s13059-015-0849-0
  • 10.1093/bioinformatics/btu153
  • 10.1093/nar/gkl791
  • 10.1128/AAC.00419-13
  • 10.1007/978-1-59745-535-0_4
  • 10.1093/nar/gkw387
  • 10.1093/nar/gks406
  • 10.1186/1471-2105-10-421
  • 10.1016/s0378-1119(01)00819-8
  • 10.1186/1471-2164-9-75
  • 10.1093/nar/gkj014
  • 10.1039/C5AY02550H
  • 10.1186/1471-2105-12-124
  • 10.1371/journal.pone.0022594
  • 10.1093/molbev/mst024
  • 10.1093/molbev/msu300
  • 10.1093/molbev/msab318
  • 10.1073/pnas.94.13.6815
  • 10.1111/j.2041-210X.2012.00196.x
  • 10.1093/oxfordjournals.molbev.a026201
  • 10.1098/rspb.2001.1862
  • (2019)
  • 10.1093/molbev/msw080
  • 10.1128/JB.00298-06
  • 10.1073/pnas.080520697
  • 10.1111/j.1462-5822.2010.01560.x
  • 10.1371/journal.pone.0023184
  • 10.1099/mic.0.2007/007609-0
  • 10.1016/j.vetmic.2009.12.004
  • 10.1089/mdr.2012.0026
  • 10.3168/jds.S0022-0302(01)74595-X
  • 10.1111/j.1574-6976.2005.00011.x
  • 10.1371/journal.pone.0040119
  • 10.1128/AEM.00661-11
  • 10.1038/emboj.2012.212
  • 10.1080/21505594.2018.1451284
  • 10.1128/AEM.06891-11
  • 10.1016/j.virusres.2016.05.021
  • 10.1038/s41598-017-02038-y
  • 10.1016/j.gene.2014.11.019
  • 10.1111/1574-6976.12067
  • 10.3389/fgene.2017.00072
  • 10.1371/journal.pone.0084796
  • 10.1016/j.meegid.2015.11.017
  • 10.1073/pnas.0906412106
  • 10.1016/j.micpath.2020.104368
  • 10.1177/0300985813511131
  • 10.1038/nrg964
  • 10.1186/gb-2007-8-5-r71
  • 10.1101/gr.6254707
  • 10.1128/genomeA.00592-16
  • 10.1093/bioinformatics/btn578
  • 10.1093/nar/gkz239
  • Wickham, (2016)
  • 10.1093/bioinformatics/btu393