Análisis de sistemas pectinolíticos bacterianos. Aislamiento y caracterización de las pectinasas PelA de "Paenibacillus" sp. BP-23 E YvpA de "Bacillus subtilis"
- Soriano Lasheras, Margarita
- Francisco I. J. Pastor Blasco Director
Defence university: Universitat de Barcelona
Fecha de defensa: 13 October 2004
- Josep Vives Rego Chair
- Pilar Díaz Lucea Secretary
- Jesús Zueco Cruz Committee member
- María Enriqueta Arias Fernández Committee member
- Montserrat Busquets Abió Committee member
Type: Thesis
Abstract
Pectins are linear polymers of alpha-D-galacturonic acid. They are found in the middle lamella and primary cell wall of plant tissues and they are degraded by pectinases. The aim of the thesis was the caracterization of pectinases with a potencial applications in the industry. Therefore, we analysed the petinolytic system of the bacterial strains "Paenibacillus" BP-23 and "Bacillus" BP-7, previously isolated from a rice field. The pectinolytic system of both strains a multiple glycanase system, some enzymes of which have been cloned and characterized. "Paenibacillus" sp BP-23 PelA and "Bacillus subtilis" YvpA, belong to pectate lyases PL3 family, and show and unusual features among pectin and pectate lyases. For this reason, we constructed several shuttles which were used to overexpres PelA in "Bacillus subtilis".