COVID-19 y estudios microbiológicos post mortem

  1. Amparo Fernández-Rodríguez
  2. Inmaculada Casas
  3. Esther Culebras
  4. Elena Morilla
  5. Marta C. Cohen
  6. Juan Alberola
Revista:
Revista española de medicina legal: órgano de la Asociación Nacional de Médicos Forenses

ISSN: 0377-4732

Año de publicación: 2020

Volumen: 46

Número: 3

Páginas: 127-138

Tipo: Artículo

DOI: 10.1016/J.REML.2020.05.007 DIALNET GOOGLE SCHOLAR lock_openAcceso abierto editor

Otras publicaciones en: Revista española de medicina legal: órgano de la Asociación Nacional de Médicos Forenses

Objetivos de desarrollo sostenible

Resumen

En este artículo se revisan los aspectos microbiológicos de la infección COVID-19 y se presentan las recomendaciones sobre los análisis que deben realizarse en casos forenses. En primer lugar se analizan las características taxonómicas del virus, su relación con la familia Coronaviridae y su estructura genética. Se presentan brevemente las características clínicas y patológicas de la infección COVID-19, así como las coinfecciones que pueden asociarse a este virus. En el diagnóstico de laboratorio se describen la PCR —técnica de elección en la fase aguda de la infección—, los estudios antigénicos y los estudios serológicos. Finalmente se detallan los principales objetivos para los estudios microbiológicos en fallecidos en relación con la pandemia COVID-19 y se describen los principales análisis microbiológicos post mortem a realizar en fallecidos en el ámbito forense. Los estudios microbiológicos deben estar dirigidos tanto a la detección del SARS-CoV-2 como a la de las coinfecciones, que también podrían contribuir a la causa de muerte.

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Información de financiación

Los autores quieren expresar su agradecimiento a los miembros del grupo ESGFOR (European Study Group of Forensic and Post-mortem Microbiology de la ESCMID, European Society of Clinical Microbiology and Infectious Diseases) por su colaboraci?n y la experiencia aportada con respecto a la pandemia de COVID-19, y especialmente a las Dras. Nihan Ziyade y Neval Elgormus (Ministry of Justice, Council of Forensic Medicine, Postmortem Microbiology Laboratory, Estambul, Turqu?a).

Financiadores

Referencias bibliográficas

  • M. Cascella, M. Rajnik, A. Cuomo, S.C. Dulebohn, R. Di Napoli Features, Evaluation and Treatment Coronavirus (COVID-19) StatPearls (2020) Disponible en: https://www-ncbi-nlm-nih-gov.sabidi.urv.cat/books/NBK554776/
  • J. Cui, F. Li, Z.L. Shi Origin and evolution of pathogenic coronaviruses Nat Rev Microbiol, 17 (3) (2019), pp. 181-192
  • Y. Chen, Q. Liu, D. Guo Emerging coronaviruses: Genome structure, replication, and pathogenesis J Med Virol, 92 (4) (2020), pp. 418-423
  • Z.W. Ye, S. Yuan, K.S. Yuen, S.Y. Fung, C.P. Chan, D.Y. Jin Zoonotic origins of human coronaviruses Int J Biol Sci, 16 (10) (2020), pp. 1686-1697
  • V.C.C. Cheng, S.K.P. Lau, P.C.Y. Woo, Y.Y. Kwok Severe acute respiratory syndrome coronavirus as an agent of emerging and reemerging infection Clin Microbiol Rev, 20 (4) (2007), pp. 660-694
  • J. Peiris, S. Lai, L. Poon, Y. Guan, L. Yam, W. Lim, et al. Coronavirus as a possible cause of severe acute respiratory syndrome Lancet, 361 (9366) (2003), pp. 1319-1325
  • A.M. Zaki, S. Van Boheemen, T.M. Bestebroer, A.D.M.E. Osterhaus, R.A.M. Fouchier Isolation of a novel coronavirus from a man with pneumonia in Saudi Arabia N Engl J Med, 367 (19) (2012), pp. 1814-1820
  • R.J. de Groot, S.C. Baker, R.S. Baric, C.S. Brown, C. Drosten, L. Enjuanes, et al. Middle East Respiratory Syndrome Coronavirus (MERS-CoV): Announcement of the Coronavirus Study Group J Virol, 87 (14) (2013), pp. 7790-7792
  • P. Gautret Middle East Respiratory Syndrome (MERS) coronavirus. What travel health advice should be given to Hajj pilgrims? Travel Med Infect Dis, 11 (5) (2013), pp. 263-265
  • P. Zhou, X.L Yang, X.G. Wang, B. Hu, L. Zhang, W. Zhang, et al. A pneumonia outbreak associated with a new coronavirus of probable bat origin Nature, 579 (7798) (2020), pp. 270-273
  • N. Zhu, D. Zhang, W. Wang, X. Li, B. Yang, J. Song, et al. A novel coronavirus from patients with pneumonia in China, 2019 N Engl J Med, 382 (8) (2020), pp. 727-733
  • G. Li, Y. Fan, Y. Lai, T. Han, Z. Li, P. Zhou, et al. Coronavirus infections and immune responses J Med Virol, 92 (4) (2020), pp. 424-432
  • Informe SARS-CoV-2 Sociedad Argentina de Virología División de la Asociación Argentina de Microbiología Disponible en: https://aam.org.ar/src/img_up/22032020.0.pdf (2020)
  • Li F. Structure Function, and Evolution of Coronavirus Spike Proteins Annu Rev Virol, 3 (1) (2016), pp. 237-261
  • M. Hoffmann, H. Kleine-Weber, S. Schroeder, N. Krüger, T. Herrler, S. Erichsen, et al. SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor Cell, 181 (2) (2020), pp. 271-280 e8
  • J.M. Abduljalil, B.M. Abduljalil Epidemiology, genome, and clinical features of the pandemic SARS-CoV-2: a recent view New Microbes New Infect, 35 (2020), Article 100672
  • Aylward, Bruce (WHO); Liang W (PRC) Report of the WHO-China Joint Mission on Coronavirus Disease 2019 (COVID-19). WHO-China Jt Mission Coronavirus Dis 2019 [Internet] 2019(February):16–24. Disponible en: https://www.who.int/docs/default-source/coronaviruse/who-china-joint-mission-on-covid-19-final-report.pdf (2020)
  • S.A. Hassan, F.N. Sheikh, S. Jamal, J.K. Ezeh, A. Akhtar Coronavirus (COVID-19): A Review of Clinical Features, Diagnosis, and Treatment Cureus, 12 (3) (2020)
  • M. Osborn, S. Lucas, R. Stewart, B. Swift, E. Youd Briefing on COVID-19 Autopsy practice relating to possible cases of COVID-19 ( 2019-nCov, novel coronavirus from China 2019 / 2020 ) R Coll Pathol, 19 (February) (2020), pp. 1-14
  • L. Carsana, A. Sonzogni, A. Nasr, R. Rossi, A. Pellegrinelli, P. Zerbi, et al. Pulmonary post-mortem findings in a large series of COVID-19 cases from Northern Italy medRxiv (2020) Jan 1; 2020.04.19.20054262
  • S. Tian, Y. Xiong, H. Liu, L. Niu, J. Guo, M. Liao, et al. Pathological study of the 2019 novel coronavirus disease (COVID-19) through postmortem core biopsies Mod Pathol. (2020), pp. 10-12
  • B. Hanley, S.B. Lucas, E. Youd, B. Swift, M. Osborn Autopsy in suspected COVID-19 cases J Clin Pathol (2020), pp. 1-4
  • Z. Xu, L. Shi, Y. Wang, J. Zhang, L. Huang, C. Zhang, et al. Pathological findings of COVID-19 associated with acute respiratory distress syndrome Lancet Respir Med, 8 (April (4)) (2020), pp. 420-422
  • S.E. Fox, A. Akmatbekov, J.L. Harbert, G. Li, J.Q. Brown Pulmonary and Cardiac Pathology in Covid-19 : The First Autopsy Series from New Orleans 1 ) Department of Pathology, LSU Health Sciences Center, New Orleans 2 ) Pathology and Laboratory Medicine Service, Southeast Louisiana Veterans Healthcare System 3 medRxiv (2020)
  • Z. Varga, A.J. Flammer, P. Steiger, M. Haberecker, R. Andermatt, A.S. Zinkernagel, et al. Endothelial cell infection and endotheliitis in COVID-19 Lancet, 395 (10234) (2020), pp. 1417-1418
  • N. Poyiadji, G. Shahin, D. Noujaim, M. Stone, S. Patel Griffith B. COVID-19–associated Acute Hemorrhagic Necrotizing Encephalopathy: CT and MRI Features Radiology, 6 (March 31 (1)) (2020), Article 201187
  • Y. Wu, X. Xu, Z. Chen, J. Duan, K. Hashimoto, L. Yang, et al. Nervous system involvement after infection with COVID-19 and other coronaviruses Brain Behav Immun (2020) Mar;(January)
  • P. Zhou, Z. Liu, Y. Chen, Y. Xiao, X. Huang, X.G. Fan Bacterial and fungal infections in COVID-19 patients: A matter of concern Infect Control Hosp Epidemiol (2020), pp. 1-2
  • T.M. Rawson, L.S.P. Moore, N. Zhu, N. Ranganathan, K. Skolimowska, M. Gilchrist, et al. Bacterial and fungal co-infection in individuals with coronavirus: A rapid review to support COVID-19 antimicrobial prescribing Clin Infect Dis, 0954162 (May 2 (478)) (2020), pp. 1-4
  • P. Koehler, O.A. Cornely, B.W. Bottiger, F. Dusse, D.A. Eichenauer, F. Fuchs, et al. COVID-19 Associated Pulmonary Aspergillosis Mycoses (2020)
  • D. Wang, B. Hu, C. Hu, F. Zhu, X. Liu, J. Zhang, et al. Clinical Characteristics of 138 Hospitalized Patients with 2019 Novel Coronavirus-Infected Pneumonia in Wuhan, China JAMA - J Am Med Assoc, 323 (11) (2020), pp. 1061-1069
  • N. Chen, M. Zhou, X. Dong, J. Qu, F. Gong, Y. Han, et al. Epidemiological and clinical characteristics of 99 cases of 2019 novel coronavirus pneumonia in Wuhan, China: a descriptive study Lancet, 395 (10223) (2020), pp. 507-513
  • D. Lin, L. Liu, M. Zhang, Y. Hu, Q. Yang, J. Guo, et al. Co-infections of SARS-CoV-2 with multiple common respiratory pathogens in infected patients Sci China Life Sci, 63 (4) (2020), pp. 606-609
  • S. Richardson, J.S. Hirsch, M. Narasimhan, J.M. Crawford, T. McGinn, K.W. Davidson, et al. Presenting Characteristics, Comorbidities, and Outcomes Among 5700 Patients Hospitalized With COVID-19 in the New York City Area JAMA (2020) Apr 22 http://dx.doi.org.sabidi.urv.cat/10.1001/jama.2020.6775
  • J. Chaung, D. Chan, S. Pada, P.A. Tambyah Coinfection with COVID-19 and Coronavirus HKU1 - the critical need for repeat testing if clinically indicated J Med Virol (2020) http://dx.doi.org.sabidi.urv.cat/10.1002/jmv.25890
  • F. Touzard-Romo, C. Tapé, J.R. Lonks Co-infection with SARS-CoV-2 and Human Metapneumovirus R I Med J (2013), 103 (2) (2020), pp. 75-76
  • X. Wu, Y. Cai, X. Huang, X. Yu, L. Zhao, F. Wang, et al. Co-infection with SARS-CoV-2 and Influenza A Virus in Patient with Pneumonia, China Emerg Infect Dis, 26 (2020) http://dx.doi.org.sabidi.urv.cat/10.3201/eid2606.200299
  • Z. Khodamoradi, M. Moghadami, M. Lotfi Co-infection of Coronavirus Disease 2019 and Influenza A: A Report from Iran Arch Iran Med, 23 (April 1 (4)) (2020), pp. 239-243
  • J. Zhao, X. Liao, H. Wang, L. Wei, M. Xing, L. Liu, et al. Early Virus Clearance and Delayed Antibody Response in a Case of Coronavirus Disease 2019 (COVID-19) With a History of Coinfection With Human Immunodeficiency Virus Type 1 and Hepatitis C Virus Clin Infect Dis, 29 (April 9 (9)) (2020), pp. 2341-2386
  • J. Chen, X. Cheng, R. Wang, X. Zeng Computed Tomography Imaging of an HIV-infected Patient with Coronavirus Disease 2019 (COVID-19) J Med Virol (2020) http://dx.doi.org.sabidi.urv.cat/10.1002/jmv.25879
  • J.L. Blanco, J. Ambrosioni, F. Garcia, E. Martínez, A. Soriano, J. Mallolas, et al. COVID-19 in patients with HIV: clinical case series Lancet HIV [Internet], 7 (May (5)) (2020), pp. e314-6
  • Documento de posicionamiento de la SEIMC sobre el diagnóstico microbiológico de COVID-19. Grupo de expertos SEIMC para el análisis del diagnóstico microbiológico del COVID-19. Junta Directiva SEIMC. Disponible en: https://seimc.org/contenidos/documentoscientificos/recomendaciones/seimc-rc-2020-Posicionamiento_SEIMC_diagnostico_microbiologico_COVID19.pdf.
  • Interpretación de las pruebas diagnósticas frente a SARS-CoV-2. versión 2.24 de abril de, Ministerio de Sanidad y Consumo, Instituto de Salud Carlos III, SEIMC (2020) Disponible en: https://www-mscbs-gob-es.sabidi.urv.cat/en/profesionales/saludPublica/ccayes/alertasActual/nCov-China/documentos/INTERPRETACION_DE_LAS_PRUEBAS.pdf
  • Y. Liu, L.M. Yan, L. Wan, T.X. Xiang, A. Le, J.-M. Liu, et al. Viral dyna-mics in mild and severe cases of COVID-19 Lancet Infect Dis (2020) http://dx.doi.org.sabidi.urv.cat/10.1016/S1473-3099(20)30232-2
  • Q. Li, X. Guan, P. Wu, X. Wang, L. Zhou, Y. Tong, et al. Early transmission dynamics in Wuhan, China, of novel coronavirus-infected pneumonia N Engl J Med., 382 (13) (2020), pp. 1199-1207
  • M.M. Arons, K.M. Hatfield, S.C. Reddy, A. Kimball, A. James, J.R. Jacobs, et al. Presymptomatic SARS-CoV-2 Infections and Transmission in a Skilled Nursing Facility N Engl J Med. (2020), pp. 1-10 http://dx.doi.org.sabidi.urv.cat/10.1056/nejmoa2008457
  • Recomendaciones de SEIMC sobre el uso de las pruebasde detección de anticuerpos. 27 Abril 2020. Disponibleen: https://seimc.org/contenidos/documentoscientificos/recomendaciones/seimc-rc-2020-Recomendaciones usode las pruebas de deteccion deanticuerpos.pdf.
  • L. Grzelak, S. Temmam, C. Planchais, C. Demeret, C. Huon, F. Guivel, et al. SARS-CoV-2 serological analysis of COVID-19 hospitalized patients, pauci-symptomatic individuals and blood donors medRxiv, 2020 (2020) 04.21.20068858
  • J. Zhao, Q. Yuan, H. Wang, W. Liu, X. Liao, Y. Su, et al. Antibody responses to SARS-CoV-2 in patients of novel coronavirus disease 2019 Clin Infect Dis (2020) Mar 28 http://dx.doi.org.sabidi.urv.cat/10.1093/cid/ciaa344
  • L. Guo, L. Ren, S. Yang, M. Xiao, D. Chang, F. Yang, et al. Profiling Early Humoral Response to Diagnose Novel Coronavirus Disease (COVID-19) Clin Infect Dis (2020), pp. 1-8
  • IDSA COVID19 Antibody Testing Primer (2020) Actualizado 29 abril 2020. Disponible en: https://www.idsociety.org/globalassets/idsa/public-health/covid-19/idsa-covid-19-antibody-testing-primer.pdf
  • T. Hoffman, K. Nissen, J. Krambrich, B. Rönnberg, D. Akaberi, M. Esmaeilzadeh, et al. Evaluation of a COVID-19 IgM and IgG rapid test; an efficient tool for assessment of past exposure to SARS-CoV-2 Infect Ecol Epidemiol [Internet], 10 (1) (2020), 10.1080/20008686.2020.1754538 Available from:
  • G. Lippi, G.L. Salvagno, M. Pegoraro, V. Militello, C. Caloi, A. Peretti, et al. Assessment of immune response to SARS-CoV-2 with fully automated MAGLUMI 2019-nCoV IgG and IgM chemiluminescence immunoassays Clin Chem Lab Med (2020)
  • J.B. Aguilar, J.S. Faust, L.M. Westafer, J.B. Gutierrez Investigating the Impact of Asymptomatic Carriers on COVID-19 Transmission medRxiv, 2020 (2020) 03.18.20037994
  • X. He, E.H.Y. Lau, P. Wu, X. Deng, J. Wang, X. Hao, et al. Temporal dynamics in viral shedding and transmissibility of COVID-19 Nat Med (2020)
  • M.A. Quail, M. Smith, P. Coupland, T.D. Otto, S.R. Harris, T.R. Connor, et al. A tale of three next generation sequencing platforms: comparison of Ion Torrent, Pacific Biosciences and Illumina MiSeq sequencers BMC Genomics, 13 (1) (2012), p. 1
  • S. Jünemann, F.J. Sedlazeck, K. Prior, A. Albersmeier, U. John, J. Kalinowski, et al. Updating benchtop sequencing performance comparison Nat Biotechnol, 31 (April 5 (4)) (2013), pp. 294-296
  • Quick J. Artic amplicon sequencing protocol for MinION for nCoV-2019. dx.doi.org/10.17504/protocols.io.bbmuik6w.
  • S. Elbe, G. Buckland-Merrett Data, disease and diplomacy: GISAID’s innovative contribution to global health Glob Challenges, 1 (1) (2017), pp. 33-46
  • F.D. Fuertes, M.I. Caballero, S. Monzón, P. Jiménez, S. Varona, I. Cuesta, et al. Phylodynamics of SARS-CoV-2 transmission in Spain bioRxiv, 2020 (2020) 04.20.050039
  • H. Su, M. Yang, C. Wan, L.X. Yi, F. Tang, H.Y. Zhu, et al. Renal histopathological analysis of 26 postmortem findings of patients with COVID-19 in China Kidney Int (2020)
  • L.M. Barton, E.J. Duval, E. Stroberg, S. Ghosh, S. Mukhopadhyay COVID-19 Autopsies, Oklahoma, USA Am J Clin Pathol, 153 (May 5 (6)) (2020), pp. 725-733
  • Y. Xiaohong, L. Tingyuan, H. Zhicheng, P. Yifang, L. Huawen, Y. Shicang, et al. A pathological report of three COVID-19 cases by minimally invasive autopsies Chinese J Pathol, 49 (4) (2020), pp. 291-293
  • S. Tian, W. Hu, L. Niu, H. Liu, H. Xu, S.-Y. Xiao Pulmonary Pathology of Early-Phase 2019 Novel Coronavirus (COVID-19) Pneumonia in Two Patients With Lung Cancer J Thorac Oncol, 15 (May (5)) (2020), pp. 700-704
  • D.E. Justicia Recomendaciones del Consejo Médico Forense con motivo de la pandemia del COVID-19. [actualizado 20 marzo 2020] (2020), pp. 4-5
  • R. Wölfel, V.M. Corman, W. Guggemos, M. Seilmaier, S. Zange, M.A. Müller, et al. Virological assessment of hospitalized patients with COVID-2019 Nature (2020), pp. 1-14
  • A. Fernández-Rodríguez, M.C. Cohen, J. Lucena, W. Van de Voorde, A. Angelini, N. Ziyade, et al. How to optimise the yield of forensic and clinical post-mortem microbiology with an adequate sampling: a proposal for standardisation Eur J Clin Microbiol Infect Dis, 34 (May 14 (5)) (2015), pp. 1045-1057
  • A. Fernández-Rodríguez, J.L. Burton, L. Andreoletti, J. Alberola, P. Fornes, I. Merino, et al. Post-mortem microbiology in sudden death: sampling protocols proposed in different clinical settings Clin Microbiol Infect, 25 (5) (2019), pp. 570-579
  • A. Fernández-Rodríguez, J. Alberola, M.C. Cohen Análisis microbiológico post mórtem Enferm Infecc Microbiol Clin, 31 (December (10)) (2013), pp. 685-691
Fuente de los datos: Dialnet