Evaluación de la bioactividad de ciprofloxacino y vancomicina incorporados en cementos óseos poliacrílicos
- Javier Martínez Moreno
- Virginia Merino
- Amparo Nácher Alonso
- Mónica Climente Martí
- José Luís Rodrigo Pérez
- Matilde Merino Sanjuán
ISSN: 1697-4298, 0034-0618
Any de publicació: 2015
Volum: 81
Número: 3
Pàgines: 239-246
Tipus: Article
Altres publicacions en: Anales de la Real Academia Nacional de Farmacia
Resum
Antibiotic loaded bone cement used in prosthesis fixing, is a local release form that minimizes the prevalence and the complications that the antibiotics would unleash when administered intravenously. The aim of this work is to study in vitro release kinetics of ciprofloxacin and vancomycin loaded in different commercial cements and evaluate the bioactivity through a simulation exercise pharmacokinetics. Samples were prepared with commercial bone cement and ciprofloxacin and vancomycin hydrochloride (40:0,5:0,5). Release study were carried out under stirring in phosphate buffer, pH=7,4, for two months at 37ºC. The antibiotic amount and elution rate, were compared using ANOVA. In order to study the bioactivity, Monte Carlo simulation was performed. The ciprofloxacin released from samples for 8 weeks was 0.29±0.06 mg from the Palacos® cements, 0.44±0.06 mg from LimaCMT1® and 0.18±0.04 mg from Simplex®. The vancomycin released for 24 hours was 0.34±0.17 mg from Palacos® cement, 0.68±0.16 mg from LimaCMT1® and 0.17±0.02 mg from Simplex®. After this time the release stopped. The simulation study shows that during the first 72 hours, the antibiotic coverage would depends on the bone cement, the sensitivity of the microorganism and postoperative day. At subsequence times, it is expected that local bioactivity increases.