miR-1226 detection in GCF as potential biomarker of chronic periodontitisa pilot study

  1. Pablo Micó Martínez 1
  2. Jose Luis Garcia Gimenez 2
  3. Marta Seco Cervera 3
  4. Andrés López Roldán 1
  5. Pedro J. Almiñana Pastor 1
  6. Francisco M. Alpiste Illueca 1
  7. Federico V. Pallardó Calatayud 2
  1. 1 Dept. of Stomatology. Faculty of Medicine and Dentistry. University of Valencia. St/ Gascó Oliag, 1, 46010. Valencia, Spain
  2. 2 Center for Biomedical Network Research on Rare Diseases (CIBERER). CIBER-ISCIII. St/ Álvaro de Bazán, 10, 46010. Valencia, Spain; Dept. of Physiology. Faculty of Medicine and Dentistry. University of Valencia. Av/ Blasco Ibañez, 15, 46010. Valencia, Spain; INCLIVA Health Research Institute. Av/ de Menendez y Pelayo, 4, 46010. Valencia, Spain; Epigenetics Research Platform. CIBERER-UV. Av/ Blasco Ibañez, 15, 46010. Valencia, Spain
  3. 3 Center for Biomedical Network Research on Rare Diseases (CIBERER). CIBER-ISCIII. St/ Álvaro de Bazán, 10, 46010. Valencia, Spain; Dept. of Physiology. Faculty of Medicine and Dentistry. University of Valencia. Av/ Blasco Ibañez, 15, 46010. Valencia, Spain; INCLIVA Health Research Institute. Av/ de Menendez y Pelayo, 4, 46010. Valencia, Spain
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Revista:
Medicina oral, patología oral y cirugía bucal. Ed. inglesa

ISSN: 1698-6946

Año de publicación: 2018

Volumen: 23

Número: 3

Páginas: 8

Tipo: Artículo

DOI: 10.4317/MEDORAL.22329 DIALNET GOOGLE SCHOLAR lock_openAcceso abierto editor

Otras publicaciones en: Medicina oral, patología oral y cirugía bucal. Ed. inglesa

Resumen

The study and identification of new biomarkers for periodontal disease, such as microRNAs (miRNAs), may give us more information about the location and severity of the disease and will serve as a basis for treatment planning and disease-monitoring. miRNAs are a group of small RNAs which are involved in gene regulation by binding to their messenger RNA target (mRNA). In this pilot study, the procedure for purifying miRNAs from gingival crevicular fluid (GCF) was, for the first time, described. In addition, the concentration of miRNAs in GCF was analyzed and compared between patients with moderate or severe chronic periodontitis (CP) and healthy controls. GCF samples were collected from single-rooted teeth of patients with moderate or severe CP (n=9) and of healthy individuals (n=9). miRNAs were isolated from GCF using miRNeasy Serum/Plasma kit (Qiagen, CA. USA). Reverse transcription polymerase chain reaction (qRT-PCR) was used to determine the expression of a series of miRNAs candidates that are related to bone metabolism. The significance of differences in miRNA levels between both groups was determined using Mann-Whitney U test. The results from this pilot study indicate that miRNAs can be isolated from GCF. Six different miRNAs were analyzed (miR-671, miR-122, miR-1306, miR-27a, miR-223, miR-1226), but only miR-1226 showed statically significant differences between the CP group and healthy controls (p<0.05). This miRNA was downregulated in patients with CP. Within the limitations of the present study, it may be concluded that miR-1226 can be a promising biomarker for periodontal disease, adding relevant information to common clinical parameters used for diagnosis and prognosis of periodontitis.

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Fuente de los datos: Dialnet