A new set of DNA macrochips for the yeast Saccharomyces cerevisiaefeatures and uses
- Trinidad M. Alberola 1
- Jose Luís García Martínez 1
- Oreto Antúnez 1
- Laia Viladevall Masbernat 2
- Anna Barceló Vernet 2
- Joaquín Ariño Carmona 2
- José Enrique Pérez Ortín 1
- 1 DNA Chips Service-SCSIE and Department of Biochemistry and Molecular Biology, University of Valencia, Spain
- 2 DNA Microchips and Sequencing Service and Department of Biochemistry and Molecular Biology, Autonomous University of Barcelona, Bellaterra, Spain
ISSN: 1618-1905
Ano de publicación: 2004
Volume: 7
Número: 3
Páxinas: 199-206
Tipo: Artigo
Outras publicacións en: International microbiology: official journal of the Spanish Society for Microbiology
Resumo
The yeast Saccharomyces cerevisiae has been widely used for the implementation of DNA chip technologies. For this reason and due to the extensive use of this organism for basic and applied studies, yeast DNA chips are being used by many laboratories for expression or genomic analyses. While membrane arrays (macroarrays) offer several advantages, for many laboratories they are not affordable. Here we report that a cluster of four Spanish molecular-biology yeast laboratories, with relatively small budgets, have developed a complete set of probes for the genome of S. cerevisiae. These have been used to produce a new type of macroarray on a nylon surface. The macroarrays have been evaluated and protocols for their use have been optimized. [Int Microbiol 2004; 7(3):199-206]