"Saccharomyces cerevisiae" Rds2 transcript factor involvement in cell wall composition and architecture
- Inmaculada Moreno Gimeno
- Nunzia Tutrone
- Rafael Sentandreu
- Eulogio Valentín Gómez
ISSN: 1618-1905
Año de publicación: 2008
Volumen: 11
Número: 1
Páginas: 57-64
Tipo: Artículo
Otras publicaciones en: International microbiology: official journal of the Spanish Society for Microbiology
Resumen
Although the cell wall is very important in yeasts, relatively little is known about the relationship between its structure and function. In Saccharomyces cerevisiae, a family of 55 transcription factor proteins unique to fungi, so-called zinc cluster proteins, has been described. Of these, Rds2 has been identified as an activator/inhibitor of gluconeogenesis. However, previous studies have pointed out additional roles for this protein, specifically, in the modulation of cell-wall architecture and drug sensitivity. In this work, evidence regarding the role of Rds2 as a regulator of cell-wall architecture and composition is presented based on phenotypical analysis of the cell walls prepared from a S. cerevisiae Rds2 mutant strain. Analyses of the sensitivity of this rds2 mutant to different drugs and to osmotic stress showed that Rds2 is indeed involved in the drug-sensitivity response and plays a role in determining osmotic sensitivity.