Efecto del tratamiento con pentoxifilina en la isquemia-reperfusión intestinal.

Abstract

A study was carried out using male Wistar rats to assess the effect of Pentoxifilina (PTX) on the intestinal lesion caused by Ischemia-Reperfusion and thus consider how suitable its use is in cases of small intestine transplant. Material and method One hundred and fifty rats were placed randomly into 6 groups awo control g4:oups, which were given physiological serum (saline solution?) and 4 groups wffich were treated with Pentoxifilina. Group P1 (1 mg/kg of PTX one hour before is'chérhia), group P2 (1 mg/kg of PTX at the moment of reperfusion), group P3 (10/kg of PTX 1 hour before ischemia), groupP4 (10mg/kg of PTX at the moment of reperfusion). The intestinal ischer,nia y,ras done by clamping the superior mesenteric artery for 120 minutes, the timé nécessary for 1 hour reperfusion. The drug used was Pentoxifilina, which is a haemorrhaging agent belonging to the mefn1ipa flámily. Its most notable properties are the increase in eritrocitary deformation decrease in blood viscosity and the increase in the fibrinolitical ability of plasma, effects which contribute to the increase in blood circulation. Tests carried out.: Study of the survival rate at 17 days Biochemical analyses :urea, creatinin, sodium, potassium, calcium, chlorine, phosphorus, LDH. Analyses of malonildialdehyde and mieloperoxidasa in the ischemic intestinal tissue, which gives us the degree of lipid peroxidation and neutrophylic infiltration, respectively. Anatomopathological study of intestinal cuts. Study of intestinal microcirculation using the Laser Doppler technique, reading the measurements without interruption since one hour before the ischemia until 1 hour after the reperfusion. Conclusions The results from this study suggest that Pentoxifilina 1.has a favourable outcome on the lesion caused by intestinal reperfusion 2. protects intestinal circulation thanks to it haemorrhaging action 3. minimizes the lipid peroxidation process 4. the protective effect of PU depends on the moment of its administration, the best timé being rrioment zero of feperfusipn 5 Its efifect 15 nbt dependent on dose in the range of treatment used THE EFFECT OF PENTOXIFILINA TREATMENT ON INTESTINAL ISCHEMIA.REPERFUSION A study was carried out using male Wistar rats to assess the effect of Pentoxifilina (PTX) on the intestinal lesion caused by Ischemia-Reperfusion and thus consider how suitable its use is in cases of small intestine transplant. Material and method One hundred and fifty rats were placed randomly into 6 groups awo control g4:oups, which were given physiological serum (saline solution?) and 4 groups wffich were treated with Pentoxifilina. Group P1 (1 mg/kg of PTX one hour before is'chérhia), group P2 (1 mg/kg of PTX at the moment of reperfusion), group P3 (10/kg of PTX 1 hour before ischemia), groupP4 (10mg/kg of PTX at the moment of reperfusion). The intestinal ischer,nia y,ras done by clamping the superior mesenteric artery for 120 minutes, the timé nécessary for 1 hour reperfusion. The drug used was Pentoxifilina, which is a haemorrhaging agent belonging to the metibcantina flámily. Its most notable properties are the increase in eritrocitary deformation the decrease in blood viscosity and the increase in the fibrinolitical ability of plasma, effects which contribute to the increase in blood circulation. Tests carried out.: Study of the survival rate at 17 days Biochemical analyses :urea, creatinin, sodium, potassium, calcium, chlorine, phosphorus, LDH . Analyses of malonildialdehyde and mieloperoxidasa in the ischemic intestinal tissue, which gives us the degree of lipid peroxidation and neutrophylic infiltration, respectively. Anatomopathological study of intestinal cuts. Study of intestinal microcirculation using the Laser Doppler technique, reading the measurements without interruption since one hour before the ischemia until 1 hour after the reperfusion. Conclusions The results from this study suggest that Pentoxifilina 1.has a favourable outcome on the lesion caused by intestinal reperfusion 2. protects intestinal circulation thanks to it haemorrhaging action 3. minimizes the lipid peroxidation process 4. the protective effect of PU depends on the moment of its administration, the best timé being rrioment zero of feperfusipn 5 Its efifect 15 nbt dependent on dose in the range of treatment used